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Cryogenic Control Systems
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Shanghai GenePharma
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Siemens AG
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Image Search Results
Journal: The Journal of Reproduction and Development
Article Title: Heat shock decreases the embryonic quality of frozen-thawed bovine blastocysts produced in vitro
doi: 10.1262/jrd.2015-003
Figure Lengend Snippet: Representative images of embryos exposed to heat shock in experiment 2. Fresh: blastocysts on day 7 (at 168 hpi) derived from the second method (M199). Cryo-con: blastocysts incubated at 38.5 C for 6 h after thawing. Cryo-HS: blastocysts cultured at 41.0 C for 6 h after thawing. Cryo-con 48 h: blastocysts incubated at 38.5 C for 48 h after thawing. Cryo-HS 48 h: blastocysts exposed at 41.0 C for 6 h and then cultured at 42 h at 38.5 C (magnification, × 100; scale bar, 200 μm). A: Shrunken (no re-expansion of blastocoel). B: Expanding (re-expanding blastocoel but not completely filling the blasotcyst and reaching the zona pellucida). C: Expanded (recovered to expanded blastocyst).
Article Snippet: There was no difference between cryo-con and cryo-HS blastocysts at 48 h. POU5F1 levels were significantly lower in
Techniques: Derivative Assay, Incubation, Cell Culture
Journal: The Journal of Reproduction and Development
Article Title: Heat shock decreases the embryonic quality of frozen-thawed bovine blastocysts produced in vitro
doi: 10.1262/jrd.2015-003
Figure Lengend Snippet: Effect of heat shock applied to frozen-thawed blastocysts on gene expression. Results are means ± SEM of the fold change relative to fresh blastocysts. Heat shock protein 70 kDa ( HSPA1A , A), interferon tau ( IFNT , B) and POU domain, class 5, transcription factor 1 ( POU5F1 , C). Cryo-con: blastocysts incubated at 38.5 C for 6 h after thawing. Cryo-HS: blastocysts incubated at 41.0 C for 6 h after thawing. Cryo-con 48 h: blastocysts incubated at 38.5 C for 48 h after thawing. Cryo-HS at 48 h: blastocysts exposed at 41.0 C for 6 h and then cultured at 38.5 C for 42 h. Different letters above bars indicate P < 0.05.
Article Snippet: There was no difference between cryo-con and cryo-HS blastocysts at 48 h. POU5F1 levels were significantly lower in
Techniques: Expressing, Incubation, Cell Culture
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression
doi: 10.1186/s13046-017-0499-z
Figure Lengend Snippet: MiR-381 suppresses gastric cancer proliferation, migration and invasion in vitro. a , b Cell count and MTT assay analysis were used to evaluate the proliferation of gastric cancer cells (AGS and BGC-823) after transfection with the miR-381 agomiR (agomiR-381) or negative control (Con). ** P < 0.01. c , d Transwell assay was performed to detect the ability of migration and invasion of agomiR-381 transfected gastric cancer cells and their negative control. ** P < 0.01. ( e , f ) Cell count and MTT assay analysis were used to evaluate the proliferation of gastric cancer cells (MKN-28 and SGC-7901) after transfection with the miR-381 antagomiR (antagomiR-381) or negative control (Con). * P < 0.05, ** P < 0.01. g , h Transwell assay was performed to detect the ability of migration and invasion of antagomiR-381 transfected gastric cancer cells and their negative control. ** P < 0.01
Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their
Techniques: Migration, In Vitro, Cell Counting, MTT Assay, Transfection, Negative Control, Transwell Assay